Fig. 4

METTL3 regulates the expression of ZFAS1. A The level of m6A modification in total RNA in clinical samples (case1 and case2), n = 3. B, C QRT-PCR and WB detect the expression of METTL3 mRNA and protein, n = 53. D The M6A methylation level of ZFAS1 in normal nasopharyngeal epithelial cells (NP69 and N2Tert) and NPC cells (HK-1 and HONE-1) was determined by MeRIP-qPCR analysis. E The change of m6A-modified ZFAS1 increased when METTL3 interfered with the expression. The input RNA fraction Ct value was used to account for RNA sample preparation differences, and negative control groups (IgG) were used to adjust background fraction. F The graph shows METTL3 and ZFAS1 expression upon METTL3 knock down. G Compared with the control, the stability of ZFAS1 RNA was reduced in HHONE-1 cells with knockout of METL3 gene. Treat the cells with 5 mg/mL Actinomycin D, and isolate RNA at 0, 2, and 4 h. *p < 0.05, compared with relative control group. NC: normal control, si-METLL31: Small interfering RNA METTL3-1, si-METLL32: Small interfering RNA METTL3-2