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Table 4 Associations between the presence of Mollicutes, H. pylori, F. nucleatum and M. hyorhinis and clinicopathological factors

From: Evaluating the presence of Mycoplasma hyorhinis, Fusobacterium nucleatum, and Helicobacter pylori in biopsies of patients with gastric cancer

Clinicopathological characteristics Cases
n = 73 (%)
Mollicutes (%) H. pylori (%) F. nucleatum (%) M. hyorhinis (%)
PCR Positive P PCR Positive P qPCR Positive P qPCR Positive P IHC Positive P
Gender            
Male 54 (74.0) 6 (11.1) 0.781 4 (7.4) 0.670 12 (22.2) 0.142 4 (7.4) 0.102 5 (8.5) a 0.446 a
Female 19 (26.0) 3 (15.8)   2 (10.5)   4 (21.1)   4 (21.1)   3 (14.3) a  
Age            
 ≤ 59 18 (24.7) 1 (5.6) 0.553 2 (11.1) 0.607 4 (22.2) 0.030 1 (5.6) 0.398 0 (0.0) a 0.108 a
 > 59 55 (75.3) 8 (14.5)   4 (7.3)   15 (27.3)   7 (12.7)   8 (12.9) a  
Tumour size (cm)            
 ≤ 4 24 (32.9) 4 (16.7) 0.430 2 (8.3) 0.980 6 (25.0) 0.053 3 (12.5) 0.768 4 (14.8) a 0.306 a
 > 4 49 (67.1) 5 (10.2)   4 (8.2)   13 (26.5)   5 (10.2)   4 (7.5) a  
TNM staging            
I/II 20 (27.4) 3 (15.0) 0.670 1 (5.0) 0.538 2 (10.0) 4.040 3 (15.0) 0.497 2 (8.7) a 0.805 a
III/IV 53 (72.6) 6 (11.3)   5 (9.4)   17 (32.1)   5 (9.4)   6 (10.5) a  
Metastasis            
Positive 41 (56.2) 7 (17.1) 0.163 6 (14.6) 0.024 12 (29.3) 0.326 6 (14.6) 0.255 5 (10.9) a 0.763 a
Negative 32 (43.8) 2 (6.3)   0 (0.0)   7 (21.9)   2 (6.3)   3 (8.8) a  
Regional lymph nodes            
NX 3 (4.1) 0 (0.0) 0.476 0 (0.0) 0.196 0 (0.0) 4.336 0 (0.0) 0.366 2 (50.0) a 0.064 a
N0 28 (38.4) 2 (7.1)   0 (0.0)   6 (21.4)   2 (7.1)   2 (6.9) a  
N1 26 (35.6) 3 (11.5)   4 (15.4)   8 (30.8)   2 (7.7)   2 (6.7) a  
N2 12 (16.4) 3 (25.0)   2 (16.7)   5 (41.7)   3 (25.0)   1 (7.7) a  
N3 4 (5.5) 1 (25.0)   0 (0.0)   0 (0.0)   1 (25.0)   1 (25.0) a  
Lymphatic invasion            
Positive 43 (58.9) 7 (16.3) 0.219 6 (13.9) 0.033 13 (30.2) 0.736 6 (13.9) 0.327 4 (8.3) a 0.543 a
Negative 30 (41.1) 2 (6.7)   0 (0.0)   6 (20.0)   2 (6.7)   4 (12.5) a  
Vascular invasion            
Positive 11 (15.1) 2 (18.2) 0.522 2 (18.2) 0.192 6 (54.5) 3.421 1 (9.1) 0.830 2 (14.3) a 0.556 a
Negative 62 (84.9) 7 (11.3)   4 (6.5)   13 (21.0)   7 (11.3)   6 (9.1) a  
Tumour type (Lauren's Class.) 3            
Intestinal 39 (53.4)) 2 (5.1) 0.110 2 (5.1) 0.028 6 (15.4) 2.609 3 (7.7) 0.564 1 (2.6) a 0.077 a
Diffuse 33 (45.2 7 (21.2)   3 (9.1)   13 (39.4)   5 (15.2)   6 (18.2) a  
Histopathological grade            
GX 1 (1.4) 0 (0.0) 0.109 1 (100.0) 0.008 10 (25.6) 1.331 0 (0.0) 0.649 0 (0.0) a 0.892 a
G1 39 (53.4) 2 (5.1)   2 (5.1)   0 (0.0)   3 (7.7)   4 (8.9) a  
G2 31 (42.5) 6 (19.4)   3 (9.7)   9 (29.0)   5 (16.1)   4 (12.5) a  
G3 2 (2.7) 1 (50.0)   0 (0.0)   0 (0.0)   0 (0.0)   2 (100.0) a  
G4 0 (0.0) 0 (0.0)   0 (0.0)   0 (0.0)   0 (0.0)   0 (0.0) a  
Haemorrhage            
Positive 30 (41.1) 3 (10.0) 0.395 3 (10.0) 0.861 8 (26.7) 0.084 3 (10.0) 0.586 4 (13.3) a 0.764 a
Negative 43 (58.9) 6 (14.0)   3 (16.3)   11 (25.6)   5 (11.6)   4 (9.3) a  
Inflammation            
Positive 17 (23.3) 3 (17.6) 0.446 3 (17.6) 0.106 5 (29.4) 0.072 2 (11.8) 0.903 2 (8.7) a 0.805 a
Negative 56 (76.7) 6 (10.7)   3 (5.4)   14 (25.0)   6 (10.7)   6 (10.5) a  
Presence of eosinophils            
Positive 13 (17.8) 3 (23.1) 0.194 2 (15.4) 0.299 4 (30.8) 0.087 2 (15.4) 0.573 1 (6.7) a 0.633 a
Negative 60 (82.2) 6 (10.0)   4 (6.7)   15 (25.0)   6 (10.0)   7 (10.8) a  
Presence of neutrophils            
Positive 14 (19.2) 2 (14.3) 0.804 2 (14.3) 0.358 2 (14.3) 2.049 1 (7.1) 0.611 1 (5.6) a 0.475 a
Negative 59 (80.2) 7 (11.9)   4 (6.8)   17 (28.9)   7 (11.9)   7 (11.3) a  
Presence of macrophages            
Positive 17 (23.3) 0 (0.0) 0.078 0 (0.0) 0.159 3 (17.6) 0.872 1 (5.9) 0.444 0 (0.0) a 0.096 a
Negative 56 (76.7) 9 (16.1)   6 (10.7)   16 (28.6)   7 (12.5)   8 (13.1) a  
Tissue necrosis            
Positive 16 (21.9) 4 (25.0) 0.081 0 (0.0) 0.176 5 (31.3) 0.621 2 (12.5) 0.823 3 (18.8) a 0.192 a
Negative 57 (78.1) 5 (8.8)   6 (10.5)   14 (24.6)   6 (10.5)   5 (7.8) a  
Papillary arrangements            
Positive 6 (8.2) 3 (50.0) 0.003 0 (0.0) 0.444 0 (0.0) 2.389 1 (16.7) 0.640 2 (28.6) a 0.086 a
Negative 67 (91.8) 6 (9.0)   6 (9.0)   19 (28.4)   7 (10.4)   6 (8.2) a  
Tumour location            
Cardia 4 (5.5) 1 (25.0) 0.703 0 (0.0) 0.365 0 (0.0) 1.698 1 (25.0) 0.759 0 (0.0) a 0.881 a
Body 25 (34.2) 4 (16.0)   4 (16.0)   6 (24.0)   2 (8.0)   3 (12.0) a  
Antrum 43 (58.9) 4 (9.3)   2 (4.7)   13 (30.2)   5 (11.6)   5 (10.0) a  
Body and Antrum 1 (1.4) 0 (0.0)   0 (0.0)   0 (0.0)   0 (0.0)   0 (0.0)  
  1. a Considering case group, n = 80
  2. qPCR, quantitative polymerase chain reaction; IHC, immunohistochemistry