|
Primer
|
Sequence (5′–3′)
|
Location
|
Amplicons
|
Ref
|
|---|
|
PC03
|
ACACAACTGTGTTCACTAGC
|
nt 1575-1594
|
110 bp
|
[29]
|
|
PC04
|
CAACTTCATCCACGTTTCAC
|
nt 1684-1669
| | |
|
GPO3
|
GGGAGCAAACACGATAGATACCCT
|
nt 221-245
|
270 bp
|
[32]
|
|
MGSO
|
TGCACCATCTGTCACTCTGTTAACCTC
|
nt 491-465
| | |
|
JW21
|
GCGACCTGCTGGAACATTAC
|
nt 691-710
|
138 bp
|
[33]
|
|
JW22
|
CGTTAGCTGCATTACTGGAGA
|
nt 829-809
| | |
|
FNF
|
CAACACCTAGTAATCATC
|
nt 2.443.126-2.443.126
|
653 bp
| |
|
FNR
|
CGAATGCTAATACCTATA
|
nt 2.443.761-2.443.778
| |
[35]
|
|
FN-Probe
|
Cy5-GGCTTCCCCATCGGCATTCC-BHQ
|
nt 2.443.229-2.443.248
|
604 bp
|
[34]
|
|
MHRHF
|
GAACGGGA TGTAGCAATACATTC
|
nt 18520-18542
| | |
|
MHRHR
|
AGCGGACTGAAGTTGAGCTTCAG
|
nt 19124-19102
| | |
|
Mhr-p37-RT-F
|
TATCTCATTGACCTTGACTAAC
|
nt 768.070-768.092
|
89 bp
|
[15]
|
|
Mhr-p37-RT-R
|
ATTTTCGCCAATAGCATTTG
|
nt 816.070-835.070
| | |
|
Mhr-p37-RT-Probe
|
6FAM-CATCCTCTTGCTTGACTACTCCTG-MGBNFQ
|
nt 774.070-796.070
| | |
- Target sequences for β-globin amplification – PC03/PC04 (110 bp). Target sequences for 16S rRNA of Mollicutes—GPO3/MGSO (270 bp), H. pylori—JW21/JW22 (138 bp), F. nucleatum—FNF/FNR (653 bp), M. hyorhinis—MHRHF/MHRHR (604 bp), and for p37 gene rRNA of M. hyorhinis—Mhr-p37-RT-F/ Mhr-p37-RT-R / Mhr-p37-RT-Probe (89 bp)
