Fig. 2

Rap1GAP is degraded by the UPP in HPV16/18 positive cervical cancer cells. Total cell lysates (as indicated) were used to detect the expression of Rap1GAP in the cells (input) and subjected to immunoprecipitation with an anti-Rap1GAP antibody. The pulled-down proteins were analyzed using immunoblotting with the anti-ubiquitin antibody to detect the interaction between Rap1GAP and ubiquitin. To avoid the disturbance bands at 55 kD and 25 kD formed by the antibody used for pull-down in co-IP, the anti-Rap1GAP antibody was from rabbit and the anti-ubiquitin antibody was from mouse in co-IP (see “Methods” section)