Fig. 2

Antigen specific antibody responses induced in vaccinated groups. Vaccination strategies are shown in Table 1 and schematically indicated at the bottom of each graph. Sera samples were collected before immunization (pre-immune) and two weeks after the last immunization (week 8). Panel (a) shows specific anti-HCV-E2 total IgG levels, Panel (b) shows isotype IgG1 levels, panel (c) shows isotype IgG2a levels and Panel (d) shows IgG2a/IgG1 ratio. All samples were measured in duplicate. Comparison of ELISA results of each immunized group with controls (pre-immune sera and PBS group) was performed using unpaired t-test. Data are represented as mean ± SD. The asterisk indicates the significant difference between values determined by one-way ANOVA or Students t-test (p < 0.05 denoted as *, p < 0.01 denoted as **, p < 0.0001 denoted as **** and ns denoted as non-significant)