Table 2 Characteristics of patients with HPV positive breast cancers in PCR nested and qPCR genotyping (Amoy test)
From: Low frequency of HPV positivity in breast tumors among patients from south-central Poland
Feature | Sample code | |
|---|---|---|
283,618 | 561,482 | |
Clinical and histopathological features | ||
Age | 46 | 56 |
Clinical stage | T2N1 | T2N2 |
Immunophenotype | Lum B HER2+ | Lum B HER2+ |
Microscopic characteristics | tightly packed nests of neoplastic cells, with central necrosis, fibrosis and calcification | loosely packed nests of neoplastic cells with areas of necrosis |
Grade | 3 tubule formation 3 points + nuclear pleomorphism 3 points + mitotic count 3 points | 3 tubule formation 3 points + nuclear pleomorphism 3 Points + mitotic count 2 points |
Tumour margin | Infiltrative | Infiltrative |
Tumour infiltrating lymphocytes | < 5% | About 10% |
Lympho-vascular involvement | Absent | Absent |
DNA extraction | ||
Total DNA concentration ± SE [µg] | 5.43 ± 0.61 | 4.30 ± 0.32 |
A260/280 | 1.74 ± 0.10 | 1.91 ± 0.11 |
A260/230 | 1.94 ± 0.10 | 1.99 ± 0.14 |
Amplification of β-actin Ct ± SE | 29.8 ± 0.4 | 29.2 ± 0.1 |
Nested PCR | ++ | ++ |
HPV genotyping by qPCR | HPV16 | HPV16 |
P16 immunostaining | Positive | Positive |