Fig. 6
From: Silencing of UBE2D1 inhibited cell migration in gastric cancer, decreasing ubiquitination of SMAD4
The increase of cell migration induced by UBE2D1 was reversed by SMAD4. A The mRNA and protein levels of SMAD4 were detected after transduction with SMAD4 overexpression lentivirus using real-time PCR and western blot. ***, P < 0.001 versus vector. B–C Transwell (B) and wound healing C assays were performed to analyze cell migration after transduction with SMAD4 and/or UBE2D1 overexpression lentiviruses in MGC-803 cells. ***, P < 0.001 versus vector; #, P < 0.05, ###, P < 0.001 versus SAMD4 + UBE2D1. D A western blot assay was performed to measure the protein levels of UBE2D1, SMAD4, MMP2, and MMP9