Fig. 5
From: Silencing of UBE2D1 inhibited cell migration in gastric cancer, decreasing ubiquitination of SMAD4
Silencing of UBE2D1 decreased the ubiquitination level of SMAD4. A UBE2D1 was identified to associate with metastasis and SMAD signaling pathways using GSEA. B Co-Ip assay was performed to detect the interaction between UBE2D1 and SMAD4. C The protein level of SMAD4 was measured after transduction with shUBE2D1 or overexpression lentivirus. D The ubiquitination level of SMAD4 was measured after transduction with shUBE2D1 lentivirus. E SMAD4 was detected after transduction with shUBE2D1 lentivirus upon treatment with 100 μg/ml CHX. F SMAD4 was detected after transduction with UBE2D1 overexpression lentivirus and/or 10 μM of MG132