Table 2 The primer sequences used for RT-qPCR
Gene | Primer |
|---|---|
HPV16 E6 | Forward: CTGCAATGTTTCAGGACCCAC Reverse: GTTGTTTGCAGCTCTGTGCAT |
DGCR8 | Forward: GTGCATGCTTGTCCCTTTGG Reverse: TGCCAACATACCTCGTAGGG |
miR-27a-3p | Forward: CAGTTCACAGTGGCTAAGTTC Reverse: CAGTTTTTTTTTTTTTTTGCGGAA |
SMG1 | Forward: AGTTAATGGAGGCCACACCC Reverse: ACTCTAAGGCTTTTACCTTTTTCAA |
GAPDH | Forward: AATCCCATCACCATCTTCC Reverse: ATCCGTTGACTCCGACCTTCAC |
U6 | Forward: GAGTCCTTCCACGATACCAA Reverse: AACGCT TCACGAATT TGC |