Table 1 Utilized primers and TaqMan probes in the present research
Target bacteria | Primer/Probe | Oligonucleotide sequence (50e30) | Size (bp) | Product size (bp) | Ref |
|---|---|---|---|---|---|
Streptococcus gallolyticus subsp. gallolyticus | Primer F Primer R Probe | CAGAAATTGGAGAGGATTTGGAAG CCGTTATTAATCACCGCTTGAC ATATCTGCTGGAATACTGTCAACATCTGCC | 24 22 30 | 85 | This study |
EPEC | Primer F Primer R Probe | AGACTACAGGAAAATCGCTTGTTAGC CGTCCCTTTGATTCCAGTTCC TTCAACATTACCGTCATCAATAGCAAGTGG | 26 21 30 | 140 | This study |
Fusobacterium nucleatum | Primer F Primer R Probe | AGCTACAAGAGAAGAAAATGAAAATGG CCAACTCCTACAAATCCAGTAACC TTACTTCATACCATACACGAGGATCTACTT | 27 24 30 | 105 | This study |
Bacteroides fragilis | Primer F Primer R Probe | CGAGGGGCATCAGGAAGAA CGGAATCATTATGCTATCGGGTA CTTGCTTTCTTTGCTGGCGACCG | 19 23 23 | 136 | [18] |
SLCO2A1 | Primer F Primer R Probe | GAGAGATTTGAATGTTGGACAAAGC ACACTTCTGTGGTCACTCGTC TCCTACTGCCATCCTTCTACCTGCCA | 25 21 26 | 89 | This study |