Table 3 Unmethylation oligonucleotide sequences and conditions for MSP
From: Host proteome linked to HPV E7-mediated specific gene hypermethylation in cancer pathways
Oligonucleotides | Sequence (5’–3’) | Amplicon (bp) | Tm (°C) |
|---|---|---|---|
C1QBP | FW: TAGTGGATTTGGTAGTGTAG | 152 | 48 |
RW: CCTAAAACACCACCAAAAAC | |||
CDKN2A | FW: TTTGAGTATTTGTTTATGGTGT | 159 | 46 |
RW: AAACACACACAATCCACC | |||
BCAP31 | FW: TTTGGTTTTTGGTTGTGGTAT | 124 | 46 |
RW: CCAACCCAACACACAAAA | |||
PTMS | FW: TGTTTTATTTTTTTTTTGTGGTTG | 128 | 46 |
RW: CAACACTACCCAACAAAAAA |