Fig. 6
From: Host proteome linked to HPV E7-mediated specific gene hypermethylation in cancer pathways
Hypermethylation of down-regulated gene expression. Agarose gel electrophoresis of promoter methylation of selected down-regulated gene candidates, C1QBP, BCAP31, CDKN2A and PTMS, which PCR product bands were showed at 152, 124, 159 and 138 bp, respectively (PCR products were amplified with met or unmet primers, which lane 1–2: methylated human control DNA, lane 3–4: unmethylated human control, lane 5–6: SiHa cell line, lane 7–8: E7-transfected HEK293, lane 9–10: EV-transfected HEK293, lane 11–12: untransfected HEK293, lane 13–14: C33A, lane 15–16: unmethylated human control DNA and “Neg” referred to negative control) (a). Percentage of methylation of gene candidate promotors. The DNA methylation of promoter of selected gene candidates (C1QBP, BCAP31, CDKN2A and PTMS) in E7–transfected HEK293 were significantly increased compared to EV-transfected HEK293, p-values of each experiment were shown above between bars (b)