Fig. 5

H-IPSE polarizes urothelial cell cycle status in a nuclear localization sequence-dependent manner. Left panel, propidium iodide-labeled HCV-29 human urothelial cells were incubated with up to 65 pmol of H03-H-IPSE and analyzed for cell cycle status. Each symbol denotes an individual culture well replicate (n = 8 for each culture condition). Middle panel, propidium iodide-labeled HCV-29 human urothelial cells were incubated with up to 65 pmol of H06-H-IPSE and analyzed for cell cycle status. Each symbol denotes an individual culture well replicate (n = 8 for each culture condition). Right panel, CFSE-labeled HCV-29 human urothelial cells were incubated with up to 65 pmol of a nuclear localization mutant of H03-H-IPSE and analyzed for cell cycle status. Each symbol denotes an individual culture well replicate