Fig. 4

H-IPSE enhances urothelial cell proliferation in a nuclear localization sequence-dependent fashion. a, H03-H-IPSE increases urothelial cell proliferation. HCV-29 human urothelial cells were incubated with 0, 43, 216, or 864 pmol of H03-H-IPSE and electrical impedance (cellular proliferation) measured in real-time using the xCELLigence platform. b, left panel, CFSE-labeled HCV-29 human urothelial cells were incubated with up to 65 pmol of H03-H-IPSE and analyzed for evidence of proliferation (decreased CFSE content per cell with each successive generation of cells). Each symbol denotes an individual culture well replicate (n = 8 for each culture condition). B, middle panel, CFSE-labeled HCV-29 human urothelial cells were incubated with up to 65 pmol of H06-H-IPSE and analyzed for evidence of proliferation. Each symbol denotes an individual culture well replicate (n = 8 for each culture condition). B, right panel, CFSE-labeled HCV-29 human urothelial cells were incubated with up to 65 pmol of a nuclear localization mutant of H03-H-IPSE and analyzed for evidence of proliferation (decreased CFSE content per cell with each successive generation of cells). Each symbol denotes an individual culture well replicate