Fig. 2

Schistosoma haematobium eggs are sufficient to induce angiogenesis and increased vascularpermeability in the bladder. a, Mice underwent bladder wall injection with S. haematobium eggs. Four days later their bladders were harvested, processed, sectioned, and stained with hematoxylin and eosin. Left and right panels show bladders injected with controlvehicle or S. haematobium eggs, respectively. Arrowheads indicate erythrocyte-containingblood vessels, which are more numerous in egg- versus vehicle-injected bladders. b, Miceunderwent bladder wall injection with S. haematobium eggs. Three weeks later mice were administered FITC-lectin to label blood vessels and Red Fluoro-Max microbeads (to measure vascular permeability) and their bladders harvested and examined by confocal microscopy. Left, middle, and right columns show FITC channel, red channel, and merged channels, respectively. Detection of red signal indicates leakage of Red Fluoro-Max microbeads out of blood vessels. Each row consists of representative images from a single mouse (n = 3 controls, n = 3 egg-injected)