Fig. 3

Hsa-miR142-3p binds to CTNNB1 3’UTR, which is interfered by lncRNA-CR594175. a 293TN cells were transfected with pGL3-wt-CTNNB1 or pGL3-mt-CTNNB1 in the presence or absence of miR142-3p-mimic or inhibitor and subjected to luciferase activity assay 48 h later. Left, predicted binding site of hsa-miR142-3p in 3′-UTR of CTNNB1; Right, effects of hsa-miR142-3p on the expression of a luciferase cassette encoding the CTNNB1 3′-UTR. The histogram shows the relative firefly luciferase activity for the different experimental groups. *, p < 0.05, and **, p < 0.01, compared with the group transfected with the same vector but without the miR142-3p mimics or miR142-3p inhibitor. b Effect of lncRNA-CR594175 silencing on regulation of CTNNB1 by hsa-miR142-3p in HepG2 cells. Left, HepG2 cells were transfected with the indicated vectors and subjected to luciferase activity assay 48 h later. The histogram shows the relative firefly luciferase activity for the different experimental groups; right, prediction of the binding sites of hsa-miR-142-3p in lncRNA-CR594175. *, p < 0.05, compared with the group transfected pGL3-wt-CTNNB1 and miR142-3p-mimics. Data are expressed as mean ± SD of at least three independent experiments