Fig. 2

Genetic intervention through a lentiviral approach. a GFP expression 72 h after HepG2 were infected with recombinant viruses Lv-shRNA-CR594175 and Lv-CTNNB1. The infection rate was estimated by dividing the number of the cells expressing GFP with the number of all the cells in each view. For statistics, five views were randomly selected, and the mean was calculated; b Evaluation of lncRNA-CR594175 and CTNNB1 levels after infection with lentiviruses. For RT-qPCR and western blotting, the β actin was used as internal reference. ** p < 0.01, vs cell group. The tests were carried out on three biological triplicates, and data are expressed as the mean ± SD