Fig. 1

Detection of expression levels of lncRNA-CR594175, hsa-miR142-3p, CTNNB1 and proteins related to Wnt pathway in adjacent normal tissue (ANT), the HCC tumors and their metastasis. a The levels of lncRNA-CR594175, hsa-miR142-3p and CTNNB1 mRNA were detected by Real-Time PCR; in the right panel the CTNNB1 protein evaluated by western blotting shows a higher level in metastatic tissues. b mRNA and protein levels of E-cadherin (95 kDa), C-myc (56 kDa), CyclinD1 (36 kDa) and MMP-9 (102 kDa) in non-tumor adjacent tissues, HCC and metastatic tissues were detected by Real-Time PCR and western blotting, respectively: representative blots; the optical density of the target band divided by the optical density of the β-actin band; Data are expressed as mean ± SD. *, p < 0.05 and **, p < 0.01, t-test