Table 2 mIHC for TAMs and PD-L1 expression on 24 BL samples (validation cohort 2) stained for PD-L1, CD68, CD163 and c-maf; mIHC for cytoxic T cells on 24 BL samples (validation cohort 2) stained for PD1, CD8 and granzyme B
mIHC for PD-1/PD-L1 expression and macrophage polarization | ||||
|---|---|---|---|---|
eBL (n = 12) | sBL (n = 8) | iBL (n = 4) | ||
TAM | M1 (CD68+/CD163-/c-maf−) | 20–40% | 30–40% | 20–30% |
M2 (CD68+/CD163+/c-maf+) | 60–80% | 60–70% | 70–80% | |
PD-L1 | TAMs (PD-L1+/CD163+) | 65–80% | 20–40% | 55–75% |
BL cells (MYC+/PD-L1+) | 10–30% | 0–10% | 0–10% | |
Exhausted cytotoxic T cells | CD8+/PD1+/granzyme B- | 60–80% | 20–40% | 60–80% |