Fig. 3

Detection of HERV-K HML-2 Xq21.33 in cell lines and African male descendants. This shows the products of amplification of DNA from two Breast cancer cell lines HCC70 and DT22 and K344 from an African American man with HIV associated lymphoma and an African American man with prostate cancer. The primer sets used march the genome of Xq21.33 using Coff Xq21.33 F to 1584 R, 2477R, 3456R and 6115R and another primer set 5355F to F Flank. The laddered bands represent the appropriate amplicon for these primers. Note that the 6115R to Coff Xq21.33 did not amplify as it is often difficult to produce a product because of the large size of the amplicon. A nonspecific amplicon is seen in the 6115 lane