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Fig. 2 | Infectious Agents and Cancer

Fig. 2

From: Is the HERV-K HML-2 Xq21.33, an endogenous retrovirus mutated by gene conversion of chromosome X in a subset of African populations, associated with human breast cancer?

Fig. 2

Panel a shows a representative agarose gel to detect homozygocity or hemizygocity in African female individuals with BrC and HERV-K HML-2 Xq21.33 virus. Amplicons from DNA from some women with BrC (#P) and a normal Gambian person are shown which were obtained using a primer in the preintegration region of Xq21.33 on the 5′ side of the virus (CoffXq21.33) and a primer on the 3′ side of the integration site (F Flank). Homozygotes would not give a product as the 9472 bp virus present in both chromosomes X will not allow amplification using this protocol whereas hemizogous (having HERV-K HML-2 Xq21.33 in only one chromosome X) would give a 480 bp product corresponding to the empty preintegration site of one chromosome X. Panel b shows a male K344 who has HIV lymphoma and has the Xq21.33 virus present. This virus is only in his X chromosome while his y chromosome has no virus. His DNA would not give a 480 bp amplicon using this same protocol. A patient with breast cancer who is hemizygous is shown in the 1st lane next to the molecular weight markers

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Infectious Agents and Cancer

ISSN: 1750-9378

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