Evolutionarily novel genes are expressed in transgenic fish tumors and their orthologs are involved in development of progressive traits in humans

Table 1 The number of zebrafish genes activated in liver transgenic tumors and expressed in liver after tumor regression, which have orthologs in the genomes of different species found by different algorithms

Organism	Species	Genome	BLASTX	PsiBlast	Ensembl89: May 2017
Organism	Species	Genome	25%, eval< 10^− 3	25%, eval< 10^− 3	25%, conf = 1
Zebrafish	Danio rerio	GRCz10, INSDC Assembly GCA_000002035.3, Sep 2014	870	870	870
Lamprey	Petromyzon marinus	Pmarinus_7.0, Jan 2011	377	522	461
Spotted Gar	Lepisosteus oculatus	GRCz10, INSDC Assembly GCA_000002035.3, Sep 2014	659	–	725
Atlantic Cod	Gadus morhua	gadMor1, Jan 2010	580	593	632
Xenopus	Xenopus tropicalis	JGI 4.2, INSDC Assembly GCA_000004195.1, Nov 2009	599	–	644
Human	Homo sapiens	GRCh38.p3, INSDC Assembly GCA_000001405.18, Dec 2013	624	593	689

As shown in Table 1, the 870 zebrafish genes have a considerable number of orthologs in the genomes of the selected species. The results obtained by different algorithms are comparable. For subsequent work, we used results obtained by Ensembl

ISSN: 1750-9378