Table 4 HPV E6/E7 gene variations and amino acid substitutions in the case and control groups
HPV | Genome | Amino | Caseb | Controlc | P value* | ||
|---|---|---|---|---|---|---|---|
positiona | acida | Mutation | Frequency(%) | Mutation | Frequency (%) | ||
HPV16 E6 case (n=23) control (n=10) | T7179G | L19V | 1 | 4.3 | 0 | 0.0 | 1.000 |
T7220G | D32E | 20 | 87.0 | 5 | 50.0 | 0.036 | |
C7377T | H85Y | 0 | 0.0 | 1 | 10.0 | 0.303 | |
G7384C | C87S | 2 | 8.7 | 0 | 0.0 | 1.000 | |
A7404T | T94S | 3 | 13.0 | 1 | 10.0 | 1.000 | |
A7484C | E120D | 1 | 4.3 | 1 | 10.0 | 0.521 | |
E7 case (n=21) control (n=10) | A7688C | N29H | 0 | 0.0 | 1 | 10.0 | 0.323 |
A7689G | N29S | 19 | 90.5 | 5 | 50.0 | 0.022 | |
C7832T | R77C | 1 | 4.8 | 1 | 10.0 | 1.000 | |
HPV31 E6 case (n=4) control (n=9) | C285T | H60Y | 0 | 0.0 | 3 | 33.3 | 0.497 |
A297G | T64A | 0 | 0.0 | 1 | 11.1 | 1.000 | |
A475G | K123R | 0 | 0.0 | 1 | 11.1 | 1.000 | |
C520T | A138V | 0 | 0.0 | 4 | 44.4 | 0.228 | |
E7 case (n=4) control (n=9) | C626T | H23K | 4 | 100.0 | 6 | 66.7 | 0.497 |
G695A | E46K | 0 | 0.0 | 4 | 44.4 | 0.228 | |
A743G | K62E | 4 | 100.0 | 9 | 100.0 | — | |
HPV33 E6 case (n=9) control (n=6) | A213C | K35N | 3 | 33.3 | 2 | 33.3 | 1.000 |
A364C | N86H | 3 | 33.3 | 2 | 33.3 | 1.000 | |
A387C | K93N | 3 | 33.3 | 1 | 16.7 | 0.604 | |
A446G | Q113R | 3 | 33.3 | 1 | 16.7 | 0.604 | |
E7 case (n=9) control (n=6) | A834G | N88D | 1 | 11.1 | 0 | 0.0 | 1.000 |
C850A | T93N | 1 | 11.1 | 0 | 0.0 | 1.000 | |
A862T | Q97L | 3 | 33.3 | 2 | 33.3 | 1.000 | |
HPV52 E6 case (n=13) control (n=17) | G108C | E3Q | 0 | 0.0 | 1 | 5.9 | 1.000 |
E7 case (n=27) control (n=26) | C624G | C24W | 0 | 0.0 | 1 | 3.8 | 0.491 |
C662T | T37I | 1 | 3.7 | 0 | 0.0 | 1.000 | |
G707A | S52D | 1 | 3.7 | 0 | 0.0 | 1.000 | |
T727G | Y59D | 1 | 3.7 | 0 | 0.0 | 1.000 | |
C733T | H61Y | 1 | 3.7 | 0 | 0.0 | 1.000 | |
G742A | D64N | 1 | 3.7 | 0 | 0.0 | 1.000 | |
T848G | L99R | 1 | 3.7 | 0 | 0.0 | 1.000 | |
HPV58 E6 case (n=25) control (n=18) | G203C | E32Q | 3 | 12.0 | 1 | 5.6 | 0.628 |
C228T | S40F | 1 | 4.0 | 0 | 0.0 | 1.000 | |
C367A | D86E | 2 | 8.0 | 2 | 11.1 | 1.000 | |
A388C | K93N | 1 | 4.0 | 6 | 33.3 | 0.015 | |
A544T | K145S | 0 | 0.0 | 1 | 5.6 | 0.419 | |
E7 case (n=12) control (n=9) | C632T | T20I | 3 | 25.0 | 3 | 33.3 | 1.000 |
G694A | G41R | 3 | 25.0 | 2 | 22.2 | 1.000 | |
C755A | T61N | 0 | 0.0 | 1 | 11.1 | 0.429 | |
G760A | G63S | 4 | 33.3 | 2 | 22.2 | 0.659 | |
G761A | G63D | 3 | 25.0 | 2 | 22.2 | 1.000 | |
A793G | T74A | 1 | 8.3 | 0 | 0.0 | 1.000 | |
C801A | D76E | 1 | 8.3 | 0 | 0.0 | 1.000 | |
T803C | V77A | 0 | 0.0 | 3 | 33.3 | 0.063 | |