Fig. 6

CRISPR/Cas9-mediated knockout of LMP1 or LMP2A down-regulates the eIF4E activation in the EBV-infected CNE-2 cells. CRISPR/Cas9-mediated knockout of LMP1 or of LMP2A was performed in the CNE-2 cells, post the infection with 10 MOI EBV, then the mRNA and the protein levels of LMP1, LMP2A, and eIF4E were examined by the method of Real-time quantitative PCR analysis (a) or of western blotting (b). Experiments were independently repeated in triplicate. ** p < 0.01; *** p < 0.001; ns, no significance