Representative case data.
a Computed tomographic examination of the neck. The thyroid cartilage is invaded by LC. b Surgical specimen of the larynx. A large transglottic tumor is noted. c–g Histology results of the primary LC lesion. Hematoxylin-eosin staining at low (c, bar = 100 μm) and high magnification (d, bar = 50 μm); p16 immunohistochemical staining (e, bar = 50 μm); DNA in situ hybridization (ISH) (f, bar = 50 μm); RNA ISH (g, bar = 20 μm). (h) p16 immunohistochemical staining in a metastatic lymph node (bar = 100 μm).