Table 3 Primer information
From: Association of host immunity with Helicobacter pylori infection in recurrent gastric cancer
Primer | Sequences of primers | Melting Temperature (°C) | Annealing Temperature (°C) |
|---|---|---|---|
IL-10F | 5′-GTG GAA GGG GAA GGT GAA-3’ | 55.8 | 60.0 |
IL-10R | 5’-CCC AAG ACT TCT CCT TGC TA-3’ | 56.3 | |
819F | 5’-GAC TCC AGC CAC AGA AGC TTA C-3’ | 60.4 | a |
819R | 5′-AGG TCT CTG GGC CTT AGT-3′ | 55.8 | |
1082A-SNP | 5’-AAC ACT ACT AAG GCT TCT TTG TGA-3’ | 57.3 | 66.5 |
1082G-SNP | 5’-AAC ACT ACT AAG GCT TCT TTG TGA GG-3’ | 58.9 | 66.5 |
819 T-SNP | 5’-TAC CCT TGT ACA GGT GAT GGA ATA-3’ | 57.1 | 57.0 |
819C-SNP | 5’-TAC CCT TGT ACA GGT GAT GGA ACA-3’ | 58.8 | 63.0 |
592A-SNP | 5′-TGA CCC CGC CGG TAC-3’ | 52.0 | 57.0 |
592C-SNP | 5′- TGA CCC CGC CGG TCC-3’ | 54.0 | 60.0 |