Quasispecies variant of pre-S/S gene in HBV-related hepatocellular carcinoma with HBs antigen positive and occult infection

Table 3 The frequencies of specific amino acid mutations associated with the major and minor population of HBV quasispecies

Region	Position	HBsAg positive (n = 10)	OBI (n = 9)	P value
Major population
S	I126S/T	30	0	0.040
	Y200F/S	0	33.3	0.025
	Y206C/H/S	30	0	0.040
Minor population
Pre-S	W4R	0	33.3	0.025
	S5A/P/T	30	0	0.040
	L30S	10	77.8	0.001
	G35R	30	0	0.040
	H51P/R	30	0	0.040
	E54K	50	0	0.006
	A60V	30	0	0.040
	W77R/Stop	30	0	0.040
	I84T	60	11.1	0.014
	N98 K/T	30	0	0.040
	G102R	30	0	0.040
	Q118R/Stop	0	44.4	0.008
	N123D	10	44.4	0.049
	S124F/P	0	33.3	0.025
S	L21F/S	70	100	0.040
	L42F/S	0	33.3	0.025
	W182Stop	30	0	0.040
	L213F/I/T	40	0	0.017

Bold indicated the amino acid position which mutational frequencies in OBI were higher than those in HBsAg positive

ISSN: 1750-9378