Quasispecies variant of pre-S/S gene in HBV-related hepatocellular carcinoma with HBs antigen positive and occult infection

Table 2 Amino acid mutations in the pre-S/S region of the major quasispecies populations

Domain	AA Position (number)	HBsAg Positive (%)	OBI (%)	P
PreS1	aa1–119 (119)	2.80 ± 1.69	4.22 ± 3.31	0.12
NTCP	aa2–48 (47)	0.60 ± 0.84	0.33 ± 0.50	0.21
HSP70	aa74–118 (45)	0.60 ± 0.70	0.33 ± 0.50	0.18
S promoter	aa66–111 (46)	0.70 ± 0.67	0.33 ± 0.50	0.10
NBS	aa103–127 (25)	0.20 ± 0.63	0.56 ± 0.88	0.16
T cell epitope	aa21–30,52–67 (26)	0.50 ± 0.71	0.67 ± 0.87	0.33
B cell epitope	aa12–47,72–78,94–117 (67)	0.70 ± 0.82	0.00 ± 0.00	0.01*
S	aa1-227 (227)	8.10 ± 8.62	7.33 ± 4.74	0.41
Pre-a determinant	aa100–119 (20)	1.20 ± 0.79	1.11 ± 0.33	0.38
a determinant	aa 124–148 (25)	0.70 ± 0.82	0.00 ± 0.00	0.01*
Post a determinant	aa 149–169 (21)	0.30 ± 0.67	0.22 ± 0.67	0.40
HLA class I	aa87–98, 186–197, 215–223 (29)	1.80 ± 1.81	1.44 ± 0.73	0.29
HLA class II	aa97–106, 171–179, 206–215 (29)	0.50 ± 0.85	0.44 ± 0.73	0.44

ISSN: 1750-9378