Fig. 5

Effects of refp17 and vp17s on viability and colony-forming capacity of MDA-MB 231 cells. a MTT assay was used to determine the viability of MDA-MB 231 cells treated or not for 48 h with refp17 or vp17s as indicated. Data represent the average of three independent experiments performed in triplicate. Statistical analysis was performed by one-way ANOVA, and the Bonferroni post-test was used to compare data. b The effect of refp17 and vp17s on breast cancer cells clonogenicity was analyzed by soft agar assay. Cells were plated in six-well plate and, after two days, the medium was replaced using fresh medium with various concentration of refp17 or vp17s (range from 50 to 200 ng/ml). Not treated cells (NT) were used as a negative control. Data represent the average number of colonies ± SD from three independent experiments performed in triplicate. The statistical significance between control and treated cultures was calculated using two-way ANOVA, and the Bonferroni post-test was used to compare data (** p < 0.01, *** p < 0.001)