Fig. 3

Effects of refp17 and vp17s on Akt, STAT3 and ERK1/2 activity in MDA-MB 231 cells. Cells were treated or not (NT) for 30 min with 50, 100 and 200 ng/ml of refp17 or vp17s and then lysed. Equal amounts of total cellular extracts were analyzed for expression of pAkt, Akt, pSTAT3, STAT3, pERK1/2 or ERK1/2 by western blot analysis using mAbs to pAkt (Ser473), Akt, pSTAT3 (Tyr705), STAT3, pERK1/2 (Thr202, Tyr204) or ERK1/2 as specific reagents. Phosphorylation of Akt, STAT3 and ERK1/2 was verified by densiometric analysis and plotting of the pAkt/Akt, pSTAT3/STAT3 and pERK1/2/ERK1/2. Upper panel, Blots from one representative experiment of three with similar results are shown. Lower panel, Values reported for Akt, STAT3 and ERK1/2 are the mean ± SD of three independent experiments. Statistical analysis was performed by one-way ANOVA, and the Bonferroni post-test was used to compare data (** p < 0.01, *** p < 0.001)