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Table 1 PCR primers used for standard and semi-nested PCR

From: High risk human papilloma viruses (HPVs) are present in benign prostate tissues before development of HPV associated prostate cancer

Primers

Primer sequence (5’➔ 3′)

Target

ß-Globin forward

GAAGAGCCAAGGACAGGTAC

ß-globin

ß-Globin reverse

CAACTTCATCCACGTTCACC

 MY11b

GCACAGGGYCAYAAYAATGG

HPV L1

 Gp6 + c

AATCATATTCCTCMMCATGTC

 Gp5+ b

TATTTGTTACTGTKGTWGATAC

HPV18 E7 forward

GACGAGCCGAACCACAAC

HPV18 E7

HPV18 E7 reverse

GGATGCACACCACGGACA

HPV16 E7 forward

AGCTCA GAGGAGGAGGATGA

HPV16 E7

HPV16 E7 reverse

GGTTTCTGAGAACAGATGGG

  1. aDegenerate bases: M = A + C, W = A + T, Y = C + T, R = A + G
  2. bForward primer
  3. cReverse primer
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Infectious Agents and Cancer

ISSN: 1750-9378

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