Table 1 PCR primer sequences used to amplify HHV8 latent and lytic mRNAs
From: Effect of electrochemotherapy on human herpesvirus 8 kinetics in classic Kaposi sarcoma
Locus | Primer name | Sequences (5′ – 3′) | cDNA Amplicon (bp) |
|---|---|---|---|
Human | |||
Il-1α | DM151 DM152 | GTCTCTGAATCAGAAATCCTTCTATC CATGTCAAATTTCACTGCTTCATCC | 421 |
HHV8 latent | |||
ORF72 | ORF72A ORF72B | CCGCGCTTTTTAACTTCTGACTCT GCTGATAATAGAGGCGGGCAATGA | 507 |
ORF73 | ORF73A ORF73B | ACTATGGAAGATTGTAGG TATATGTGTATTGTCAGAAC | 106 |
HHV8 lytic | |||
K2 | ORFK2F ORFK2R | ATTGAGTCTCTGAATGAG TTCAAGTTGTGGTCTATC | 155 |
K8 | ORFK8F ORFK8R | AAAGCATACACAAGACAG AAATAATCTGTTCCTTATGTG | 100 |
K8.1 | ORFK8.1F ORFK8.1R | CCGATGCCTTAATATCAG TTCCTCTAGTCGTTGTAG | 155 |
K10.5/K10.6 | VIRF3F VIRF3R | GAGTTTTCACCCACAAATG CAGGACTCACCTACACAG | 107 |
K10/K10.1 | VIRF4F VIRF4R | TGTTCAGTCGTTATATCA TATTCAGTGTCTGTTGTC | 182 |
ORF16 | ORF16F ORF16R | TGGCTATACTGACCTTTG GCTTCATACGCATATACAG | 78 °C |