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Fig. 4 | Infectious Agents and Cancer

Fig. 4

From: LPS promotes resistance to TRAIL-induced apoptosis in pancreatic cancer

Fig. 4

Co-stimulation with TRAIL and LPS significantly affected the impact of TRAIL on TRAIL-receptor-expression in BxPC-3 cells. Cultures of BxPC-3 were stimulated with 300 ng/ml TRAIL, 1 μg/ml LPS and 300 ng/ml TRAIL + 1 μg/ml LPS for 24 h. Non-stimulated cell cultures served as controls. The concentration of OPG within the supernatants of respective cell cultures was measured by ELISA. The expression of the TRAIL-receptors 1–4 was determined via FACS analysis. a The expression of the TRAIL-receptors 1–4 by BxPC-3 cells was measured by FACS analysis and is depicted as mean fluorescence intensity. TRAIL-stimulation highly significantly decreased the expression of TRAIL-receptor 1 (DR4) on BxPC-3 cells. N ≥ 6/group. b Representative histograms are shown. c Concentrations of OPG within the supernatants of BxPC-3 cultures are depicted. Neither stimulation with TRAIL alone nor co-stimulation with TRAIL and LPS significantly altered the levels of OPG. N = 8/group. Means and standard errors of the mean are shown. *) p < 0.05; **) p < 0.01; ***) p < 0.001

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