Fig. 3From: LPS promotes resistance to TRAIL-induced apoptosis in pancreatic cancerCo-stimulation of COLO357 cultures with LPS and TRAIL increased OPG-levels. Cultures of COLO357 were stimulated with 300 ng/ml TRAIL, 1 μg/ml LPS and 300 ng/ml TRAIL + 1 μg/ml LPS for 24 h. Non-stimulated cell cultures served as controls. The concentration of OPG within the supernatants of respective cell cultures was measured by ELISA. The expression of the TRAIL-receptors 1–4 was determined via FACS analysis. a The expression of respective TRAIL-receptors was determined by FACS analysis. Data are expressed as mean fluorescence intensity. N = 5/group. b Concentrations of OPG within the supernatants of COLO357 cultures are depicted. Whereas TRAIL-stimulated cultures displayed a trend toward decreased OPG-levels, co-stimulation with LPS and TRAIL increased OPG-levels when compared to non-stimulated controls. N = 6/group. Means and standard errors of the means are shown. **) p < 0.01Back to article page