Table 1 Sequences of probes and primers
Name of gene | Sequence of primers and probes | Fluorophores |
|---|---|---|
JAK1 | Probe: 5′AGCAGTCAGTGTGGCGTCATTCTCC-3′ Forward primer 5′- CAATTGGCATGGAACCAACGAC-3′ Reverse primer 5′-CAAATCATACTGTCCCTGAGCAAAC-3′ | 5′ FAM- 3′ BHQ-1 |
STAT1 | Probe: 5′-CGCTCTGCTGTCTCCGCTTCCACTCC-3′ Forward primer: 5′GTTGCTGAATGTCACTGAACTTACC-3′ Reverse primer: 5′- AGCTGATCCAAGCAAGCATTGG-3′ | 5′ FAM- 3′ BHQ-1 |
OAS3 | Probe 5′- AGCCTGGTGCCTGCCTTCAATGTCC-3′ Forward primer: 5′-TCCGCCTGACATCCGTAGATC-3′ Reverse primer: 5′-TCCTCCGCAGCTCTGTGAAG-3′ | 5′ FAM- 3′ BHQ-1 |
GAPDH | Probe: 5′- CCGTTGACTCCGACCTTCACCTTCC-3′ Forward primer: 5′- AGCCACATCGCTCAGACACC-3′ Reverse primer: 5′- TGACCAGGCGCCCAATACG-3′ | 5′HEX- 3′TAMRA |