Fig. 3
AFB1 inhibits the mRNA expression levels of the JAK1 (a), STAT1 (b) and OAS3. c. HepG2 cells were cultured at density of 5 × 104 cells per well of the 6-well plate until they reached 80% confluence. The cells were stimulated with or without rIFN-α (400 IU/ml) and simultaneously treated with or without AFB1 (10 μM) for 24 h. Total RNA was extracted and reverse transcribed to cDNA using random primers. Quantitative PCR was performed with gene specific primers and probes for JAK1, STAT1 and OAS3 respectively. The relative levels of JAK1, STAT1 and OAS3 after normalization to GAPDH (endogenous control) was plotted. The relative levels of JAK1, STAT1 and OAS3 were calculated using the 2-∆∆Ct (Livak) method. These results are presented as mean and standard deviations of three independent experiments each performed in triplicate wells; JAK1 (p-value ≤ 0.0001), STAT1 (p-value ≤ 0.03) and OAS3 (p-value ≤ 0.05)