Table 1 Primers used for HPV detection and genotyping
From: Human papillomavirus genotypes distribution in cervical cancer cases in Gabon
Primers | Sequences (5′ to 3′) | Target gene | Amplicons length |
|---|---|---|---|
PC04 | CAA CTT CAT CCA CGT TCA CC | β-globin | 268 bp |
GH20 | GAA GAG CCA AGG ACA GGT AC | ||
MY09 | CGT CCM ARR GGA WAC TGA TC | L1 | 450 bp |
MY11 | GCM CAG GGW CAT AAY AAT GG | ||
GP5+ | TTT GTT ACT GTG GTA GAT ACT AC | L1 | 142 bp |
GP6+ | GAA AAA TAA ACT GTA AAT CAT ATT C | ||
M = A + C, R = A + G, W = A + T, Y = C + T |