Fig. 4

Genetic rearrangement of the immunoglobulin κ chain as analyzed by the BioMed2 method. a SPEL cell line. b Peripheral blood mononuclear cells (PBMC) from another unrelated patient. Fragment analysis of PCR products of the IGK tube A using the BioMed 2 method is shown. PCR products of Vk1f/6/Vk7-Jk, Vk3f-Jk, and Vk2f/Vk4/Vk5-Jk were observed in the 120–160, 190–210, and 260–300 bp regions, respectively. Arrows indicate clonal bands. Arrow heads indicate products of the germ bands in each region