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Fig. 1 | Infectious Agents and Cancer

Fig. 1

From: Human herpesvirus multiplex ddPCR detection in brain tissue from low- and high-grade astrocytoma cases and controls

Fig. 1

Characterization of the HHV-6A, HHV-6B, CMV, EBV and RPP30 multiplex ddPCR assays. A CMV plasmid was used as a positive control to evaluate each CMV primer/probe set, with results shown in (a and b). a The ddPCR-adapted CMV (20X) primer/probe set used in this study [24] is shown on the FAM channel; c A second ddPCR-adapted CMV (20X) primer/probe set [28] that was evaluated but not used in this study is shown on the FAM channel; b HHV-6A and HHV-6B infected cells were used as positive controls in this multiplex characterization. HHV-6A (20X) and CMV (10X) are on the FAM channel and HHV-6B (20X) is on the VIC channel. Droplets containing multiple DNA targets are double positive for FAM and VIC (shown in the upper right quadrant); d An EBV transformed lymphoblastoid cell line was used as a positive control in this multiplex characterization. EBV is shown on the FAM channel and the housekeeping gene RPP30 is shown on the VIC channel

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