Table 1 Primer sequences for PCR analysis
From: H. pylori infection is related to mitochondrial microsatellite instability in gastric carcinogenesis
Repeat sequence | mtDNA region | Position | Annealing(°C) | Primer(5’-3’) |
|---|---|---|---|---|
(C)n | 270-425 | D-loop | 58 | TCCACACAGACATCAATAACA |
AAAGTGCATACCGCCAAAAG | ||||
(CA)n | 467-556 | D-loop | 55 | CCCATACTACTAATCTCATCAA |
TTTGGTTGGTTCGGGGTATG | ||||
(C)6 | 3529-3617 | ND1 | 55 | CCGACCTTAGCTCTCACCAT |
AATAGGAGGCCTAGGTTGAG | ||||
(A)7 | 4555-4644 | ND2 | 55 | CCTGAGTAGGCCTAGAAATAAA |
ACTTGATGGCAGCTTCTGTG | ||||
(T)7 | 9431-9526 | C0III | 55 | CCAAAAAGGCCTTCGATACG |
GCTAGGCTGGAGTGGTAAAA | ||||
(C)6 and(A)8 | 12360-12465 | ND5 | 55 | CACCCTAACCCTGACTTCC |
GGTGGATGCGACAATGGATT | ||||
(CCT)3 and(AGC)3 | 12940-13032 | ND5 | 55 | GCCCTTCTAAACGCTAATCC |
TCAGGGGTGGAGACCTAATT |