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Table 3 H28 HPV positive results and semi-quantification of HPV-DNA load, stratified on delay between sampling and laboratory processing of DNA extraction (2 to ≥4 weeks)

From: Use of swabs for dry collection of self-samples to detect human papillomavirus among Malagasy women

HPV positive results

HPV-DNA loada

p value

+

++

+++

Total

 

N (%)

N (%)

N (%)

N (%)

 

2 weeks

     

 High-riskb

20 (44.4)

25 (55.7)

0

45 (100.0)

0.009

 Low-riskc

9 (34.6)

12 (46.2)

5 (19.2)

26 (100.0)

0.009

3 weeks

     

 High-riskb

22 (37.9)

30 (51.7)

6 (10.3)

58 (100.0)

0.919

 Low-riskc

11 (34.4)

18 (56.3)

3 (9.4)

32 (100.0)

0.919

≥4 weeks

     

 High-riskb

38 (30.4)

75 (60.0)

12 (9.6)

125 (100.0)

0.409

 Low-riskc

20 (21.0)

58 (66.7)

9 (10.3)

87 (100.0)

0.491

TOTAL

120 (32.2)

218 (58.5)

35 (9.4)

373 (100.0)

 
  1. Abbreviations: N number
  2. a + (low), positive >40 PCR cycles, <102 copies/reaction; ++ (medium) positive within 31 to 39 PCR cycles, ≥102 and < 105 copies/reaction; +++ (high) positive before 31 PCR cycles, ≥ 105 copies/reaction
  3. bPositivity for a high-risk HPV type (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 69, 73 and 82)
  4. cPositivity for a low-risk type (6, 11, 40, 42, 43, 44, 54, 61 and 70)
  5. NB: For multiple infections each single positive result was counted for high- and low-risk types