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Table 2 H28 HPV results and semi-quantification of ß-globin DNA load, stratified on delay between sampling and laboratory processing of DNA extraction (2 to ≥4 weeks)

From: Use of swabs for dry collection of self-samples to detect human papillomavirus among Malagasy women

HPV results

ß-globin DNA loada

p value

Undetectable

+

++

+++

Total

 

N (%)

N (%)

N (%)

N (%)

N (%)

 

2 weeks

      

 Positive

1 (2.3)

1 (2.3)

32 (74.4)

9 (20.9)

43 (100.0)

<0.001

  High-riskb

0

1 (3.3)

23 (76.7)

6 (20.0)

30 (100.0)

  Low-riskc

1 (4.6)

0

17 (77.3)

4 (18.2)

22 (100.0)

 Negative

0

0

26 (65.0)

14 (35.0)

40 (100.0)

 Invalid

3 (60.0)

2 (40.0)

0

0

5 (100.0)

3 weeks

      

 Positive

2 (3.5)

4 (7.0)

38 (66.7)

13 (22.8)

57 (100.0)

<0.001

  High-riskb

1 (2.3)

4 (9.1)

30 (68.2)

9 (20.5)

44 (100.0)

  Low-riskc

1 (3.9)

2 (7.7)

18 (69.2)

5 (19.2)

26 (100.0)

 Negative

0

0

50 (72.5)

19 (27.5)

69 (100.0)

 Invalid

5 (38.5)

8 (61.5)

0

0

13 (100.0)

≥4 weeks

     

<0.001

 Positive

4 (3.7)

6 (5.5)

79 (72.5)

20 (18.4)

109 (100.0)

  High-riskb

2 (2.4)

5 (6.1)

57 (69.5)

18 (22.0)

82 (100.0)

  Low-riskc

3 (4.6)

3 (4.6)

51 (78.5)

8 (12.3)

65 (100.0)

 Negative

0

0

62 (72.9)

23 (27.1)

85 (100.0)

 Invalid

20 (71.4)

8 (28.6)

0

0

28 (100.0)

TOTAL

35 (7.8)

29 (6.5)

287 (63.9)

98 (21.8)

449 (100.0)

 
  1. Abbreviations: N number
  2. aUndetectable, absence of signal; + (low), positive >40 PCR cycles, <102 copies/reaction; ++ (medium) positive within 31 to 39 PCR cycles, ≥102 and < 105 copies/reaction; +++ (high) positive before 31 PCR cycles, ≥ 105 copies/reaction
  3. bPositivity for a high-risk HPV type (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 69, 73 and 82)
  4. cPositivity for a low-risk type (6, 11, 40, 42, 43, 44, 54, 61 and 70)
  5. NB For multiple infections positive results were pooled and a single result was generated