Figure 6From: Inhibitory effect of piperine on Helicobacter pylori growth and adhesion to gastric adenocarcinoma cellsImmunoblotting of H. pylori 60190 whole cell antigen. H. pylori was treated with various concentrations of piperine and protein levels of flagellar molecules were analyzed by immunoblotting. 1; H. pylori – vehicle control, 2; H. pylori treated with 50 μM of piperine, 3; H. pylori treated with 75 μM of piperine, 4; H. pylori treated with 100 μM of piperine. Flagellar hook component protein FlgE (77 KDa) and an integral component of the flagellar export apparatus FlhA (81 KDa) was separated by 7.5% SDS-PAGE and expression were diminished in piperine treatment in a dose dependent manner. Other proteins remained unchanged. The representative of three independent experiments.Back to article page