Figure 3
From: Loss of nuclear PTEN in HCV-infected human hepatocytes
Viral small non-coding RNA in HCV replicating cells. (A) Expression of vmr11 RNA: 1 × 105 PPH cells were transfected with 1 μg of HCV1a genomic RNA and harvested on days 3, 6 and 12 post-transfection; vmr11 was monitored by qRT-PCR (normalized to miR-16, which does not change with viral infection). (B) MicroRNA-141 expression in cells transfected with HCV genomic RNA: qRT-PCR analysis of miR-141 in PPH cells transfected with increasing amounts of viral genomic RNA. (C) vmr11 expression with increasing HCV genomic RNA: qRT-PCR of vmr11 RNA in PPH cells transfected with increasing amounts of input viral genomic RNA (0.5, 1 and 2 μg) was analyzed five days post-transfection.