Figure 2

Detection of HMTV- env gene sequences in P2 RNA. cDNA synthesized from high speed pellet (P2) RNA using polydT as primer, PCR was performed using primers 5 L and 3 L and nested PCR using primers 1XXX and 3 F as described in Methods. A. Diagram of HMTV viral RNA structure and location of the primers. B. HMTV-env cDNA sequence was amplified using primers 1XXX and 3 F in a nested PCR reaction (GenBank KJ831810). C. 2% agarose gel electrophoresis of HMTV-env 251 bp amplicon. Lane 1: HMTV-env positive sample. Lane 2: 1 kb plus DNA ladder. Lane 3: Southern blot hybridization using ³²P end labeled probe for HMTV-env positive sample.