Figure 1From: Human Mammary Tumor Virus (HMTV) sequences in human milkDetection of HMTV- env gene sequences in milk cells. PCR was performed using primers 5 L and 3 L and nested PCR using primers 1XXX and 3 F as described in Methods. A. Diagram of HMTV proviral DNA structure and location of the primers. B. HMTV-env DNA sequence using primers 1XXX and 3 F in a nested PCR reaction (GenBank KJ831809). C. 2% agarose gel electrophoresis of HMTV-env 251 bp amplicon. Lane 1: HMTV-env positive sample, Lane 2: HMTV-env positive control, Lane 3: 1 kb plus DNA ladder. Southern blot hybridization using 32P labeled probe as described in Methods. Lane 4: HMTV-env positive sample. Lane 5: HMTV-env positive DNA control.Back to article page