Table 1 List of antibodies and their respective concentrations
Primary antibody | Dilution | Company |
|---|---|---|
CD20 (IHC) | RTU | Novocastra- Leyca system, Milan (Italy) |
CD10 (IHC) | RTU | Novocastra- Leyca system, Milan (Italy) |
Bcl-6 (IHC) | RTU | Novocastra- Leyca system, Milan (Italy) |
Bcl-2 (IHC) | RTU | Novocastra- Leyca system, Milan (Italy) |
CD38 (IHC) | RTU | Novocastra- Leyca system, Milan (Italy) |
CD44 (IHC) | RTU | Novocastra- Leyca system, Milan (Italy) |
IgM (IHC) | 1:100 | Dako, Milan (Italy) |
Ki-67 (IHC) | 1:100 | Novocastra- Leyca system, Milan (Italy) |
p80 (IHC) | 1:10 | Abcam, Cambridge (UK) |
gp130 (IHC) | 1:50 | Abcam, Cambridge (UK) |
PTEN (IHC) | 1:50 | Dako, Milan (Italy) |
WT-1 (IHC) | RTU | Novocastra- Leyca system, Milan (Italy) |
PTEN (WB) | 1:500 | Dako, Milan (Italy) |
gp130 (WB) | 1:500 | Abcam, Cambridge (UK) |
p80 (WB) | 1:500 | Abcam, Cambridge (UK) |
phospho IκB-α (WB) | 1:1000 | Cell Signaling, Monza, Italy |
Phosphor Akt (WB) | 1:500 | Cell Signaling, Monza, Italy |
EBNA1 (WB) | 1:100 | Santa Cruz Biotechnology, Santa Cruz, CA, USA |
Actin (WB) | 1:1000 | Abcam, Cambridge, UK. |