Figure 4

HHV8 presence in tumor biopsy and peripheral blood. (A) Total DNA extracted from biopsy tissue (T) was analyzed by PCR for HHV8 ORF50 and ORF26 genes. Amplification of the house-keeping β-actin gene was included as a control, and DNA from a T cell line negative for HHV8 (JJhan cells) and from a PEL-derived B cell line latently infected by HHV8 (BCBL-1 cells) were respectively used as negative (C-) and positive (C+) controls. Amplification controls also included: blanks of extraction (B₁) and amplification (B₂). (B) Total DNA extracted from PBMCs (P) and plasma (P_L) fractions were analyzed as described for tumor biopsy. (C) Total RNA extracted from PBMCs was analyzed by RT-PCR for HHV8 lytic (ORF50, ORF26) and latent (ORF73) genes. Negative (C-) and positive (C+) controls, and β-actin control with (RT+) or without (RT-) previous retrotranscription are also shown.