Figure 4

Confirmation of agreement between gel electrophoresis and sequencing result. Patient BL26 and BL28 contained the full-length LMP-1 product, while BL27, BL29, and BL30 contained deletion variants by both electrophoresis and sequencing. Part a is a sample gel electrophoresis image from a PCR amplification of five eBL patient LMP-1 sequences. Lane 1 is a 100 base pair ladder, with 500 base pairs highlighted. Lane 2 is from patient BL26, lane 3 is from BL27, lane 4 is from BL28, lane 5 is from BL29, lane 6 is from BL30. Lane 7 is a no template PCR control. Part b represents the sequence traces of the corresponding eBL patient samples flanking the 30 base pair deletion.