Treatment of BC-3 cells leads to induction of ER stress markers. KSHV PEL cells (BC-3) were treated with DMSO, 20nM bortezomib (20nm BZ), 1mM thapsigargin (1mM TG) or 72uM nelfinavir (72uM NFV). RNA was isolated and RT-PCR was performed using primers for ATF-4 and XBP-1(s). Primers for β-actin were used as an internal control. Error bars indicate SEM.